Recombineering: versatile techniques to generate recombinant DNA in pro- and eukaryotic cells
Addgene: pE-FLP
E. coli knockout - CSBLwiki
CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing | Nature Communications
Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA sequences, including whole metabolic pathways, onto the Escherichia coli chromosome at well-characterised loci | Microbial Cell Factories | Full Text
Addgene: pE-FLP
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products | PNAS
Protocol to identify the core gene supported by an essential gene in E. coli bacteria using a genome-wide suppressor screen - ScienceDirect
Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA sequences, including whole metabolic pathways, onto the Escherichia coli chromosome at well-characterised loci – topic of research paper in Biological sciences. Download scholarly
Frontiers | Identification of Genes Essential for Antibiotic-Induced Up-Regulation of Plasmid-Transfer-Genes in Cephalosporin Resistant Escherichia coli
Freegenes > pE-FLP | BBF10K_000016
Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes | Protocol (Translated to German)
Antibiotic marker modifications of λ Red and FLP helper plasmids, pKD46 and pCP20, for inactivation of chromosomal genes using PCR products in multidrug-resistant strains - ScienceDirect
Fast and antibiotic free genome integration into Escherichia coli chromosome | Scientific Reports
Position and orientation of protospacers on plasmid DNA and analysis of... | Download Scientific Diagram
Addgene: pFLP-hyg
E. coli knockout - CSBLwiki
Tools for Characterization of Escherichia coli Genes of Unknown Function | Journal of Bacteriology
Addgene: pJUMP18-cI-ts_O
Gene deletion by λ-red mediated recombination using pKD4, pKD46, and... | Download Scientific Diagram